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Comparation of myogenic capacity differences in Langshan chickens with different percentage of breast muscle yield. (A) Representative images of PAX7 and <t>MYOD1</t> co-localization (scale bar = 50 μm). (B) Number of PAX7 + satellite cells (SCs) per unit area. (C) Number of MYOD1 + SCs per unit area. (D) Percentage of PAX7 + / MYOD1 + and PAX7 + /MYOD1 + cells among Pax7+ SCs. (E) Relative mRNA expression of myogenic regulatory factors. LPB: Low percentage of breast muscle yield group; HPB: High percentage of breast muscle yield group. Data were expressed as the mean ± SEM, n = 7. * P < 0.05, ** P < 0.01, *** P < 0.001.
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Comparation of myogenic capacity differences in Langshan chickens with different percentage of breast muscle yield. (A) Representative images of PAX7 and <t>MYOD1</t> co-localization (scale bar = 50 μm). (B) Number of PAX7 + satellite cells (SCs) per unit area. (C) Number of MYOD1 + SCs per unit area. (D) Percentage of PAX7 + / MYOD1 + and PAX7 + /MYOD1 + cells among Pax7+ SCs. (E) Relative mRNA expression of myogenic regulatory factors. LPB: Low percentage of breast muscle yield group; HPB: High percentage of breast muscle yield group. Data were expressed as the mean ± SEM, n = 7. * P < 0.05, ** P < 0.01, *** P < 0.001.
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Comparation of myogenic capacity differences in Langshan chickens with different percentage of breast muscle yield. (A) Representative images of PAX7 and <t>MYOD1</t> co-localization (scale bar = 50 μm). (B) Number of PAX7 + satellite cells (SCs) per unit area. (C) Number of MYOD1 + SCs per unit area. (D) Percentage of PAX7 + / MYOD1 + and PAX7 + /MYOD1 + cells among Pax7+ SCs. (E) Relative mRNA expression of myogenic regulatory factors. LPB: Low percentage of breast muscle yield group; HPB: High percentage of breast muscle yield group. Data were expressed as the mean ± SEM, n = 7. * P < 0.05, ** P < 0.01, *** P < 0.001.
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Comparation of myogenic capacity differences in Langshan chickens with different percentage of breast muscle yield. (A) Representative images of PAX7 and <t>MYOD1</t> co-localization (scale bar = 50 μm). (B) Number of PAX7 + satellite cells (SCs) per unit area. (C) Number of MYOD1 + SCs per unit area. (D) Percentage of PAX7 + / MYOD1 + and PAX7 + /MYOD1 + cells among Pax7+ SCs. (E) Relative mRNA expression of myogenic regulatory factors. LPB: Low percentage of breast muscle yield group; HPB: High percentage of breast muscle yield group. Data were expressed as the mean ± SEM, n = 7. * P < 0.05, ** P < 0.01, *** P < 0.001.
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Comparation of myogenic capacity differences in Langshan chickens with different percentage of breast muscle yield. (A) Representative images of PAX7 and MYOD1 co-localization (scale bar = 50 μm). (B) Number of PAX7 + satellite cells (SCs) per unit area. (C) Number of MYOD1 + SCs per unit area. (D) Percentage of PAX7 + / MYOD1 + and PAX7 + /MYOD1 + cells among Pax7+ SCs. (E) Relative mRNA expression of myogenic regulatory factors. LPB: Low percentage of breast muscle yield group; HPB: High percentage of breast muscle yield group. Data were expressed as the mean ± SEM, n = 7. * P < 0.05, ** P < 0.01, *** P < 0.001.

Journal: Poultry Science

Article Title: Simultaneous improvement of breast muscle yield and meat quality in Langshan chickens

doi: 10.1016/j.psj.2026.106552

Figure Lengend Snippet: Comparation of myogenic capacity differences in Langshan chickens with different percentage of breast muscle yield. (A) Representative images of PAX7 and MYOD1 co-localization (scale bar = 50 μm). (B) Number of PAX7 + satellite cells (SCs) per unit area. (C) Number of MYOD1 + SCs per unit area. (D) Percentage of PAX7 + / MYOD1 + and PAX7 + /MYOD1 + cells among Pax7+ SCs. (E) Relative mRNA expression of myogenic regulatory factors. LPB: Low percentage of breast muscle yield group; HPB: High percentage of breast muscle yield group. Data were expressed as the mean ± SEM, n = 7. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The primary antibodies used in the immunofluorescence experiment were as follows: mouse anti-PAX7 monoclonal antibody (DSHB, IA), rabbit anti-MYOD1 polyclonal antibody (Affinity Biosciences, Melbourne, Australia).

Techniques: Expressing

Untargeted metabolomic analysis of breast muscle in Langshan chickens with different percentage of breast muscle yield. (A) Principal component analysis of samples from HPB and LPB groups. (B) Volcano plot showing differential metabolites. (C-D) Bubble plot showing the top 30 up-regulated or down-regulated differential metabolites in the HPB group. (E) Mulberry plot showing top 10 KEGG pathway enriched for up-regulated or down-regulated differential metabolites. (G) Correlation analysis of muscle fiber characteristics, and differential metabolites. (H) Relative mRNA expression of lipid metabolism–related genes. (I) Relative mRNA expression of proliferation-related genes ( PAX7, MYF5, PCNA, CCND1, and CDK2 ) after 24 h treatment of different concentrations of DPA in growth medium. (J) Representative morphological images of myotube formation following 48 h of differentiation with different concentrations of DPA treatment. (K) Relative mRNA expression of myogenic differentiation–related genes ( MYOD1, MYOG, MEF2C, and MHC ) after 48 h of differentiation. LPB: Low percentage of breast muscle yield group; HPB: High percentage of breast muscle yield group. Data were expressed as the mean ± SEM, n = 7. * P < 0.05, ** P < 0.01, *** P < 0.001.

Journal: Poultry Science

Article Title: Simultaneous improvement of breast muscle yield and meat quality in Langshan chickens

doi: 10.1016/j.psj.2026.106552

Figure Lengend Snippet: Untargeted metabolomic analysis of breast muscle in Langshan chickens with different percentage of breast muscle yield. (A) Principal component analysis of samples from HPB and LPB groups. (B) Volcano plot showing differential metabolites. (C-D) Bubble plot showing the top 30 up-regulated or down-regulated differential metabolites in the HPB group. (E) Mulberry plot showing top 10 KEGG pathway enriched for up-regulated or down-regulated differential metabolites. (G) Correlation analysis of muscle fiber characteristics, and differential metabolites. (H) Relative mRNA expression of lipid metabolism–related genes. (I) Relative mRNA expression of proliferation-related genes ( PAX7, MYF5, PCNA, CCND1, and CDK2 ) after 24 h treatment of different concentrations of DPA in growth medium. (J) Representative morphological images of myotube formation following 48 h of differentiation with different concentrations of DPA treatment. (K) Relative mRNA expression of myogenic differentiation–related genes ( MYOD1, MYOG, MEF2C, and MHC ) after 48 h of differentiation. LPB: Low percentage of breast muscle yield group; HPB: High percentage of breast muscle yield group. Data were expressed as the mean ± SEM, n = 7. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The primary antibodies used in the immunofluorescence experiment were as follows: mouse anti-PAX7 monoclonal antibody (DSHB, IA), rabbit anti-MYOD1 polyclonal antibody (Affinity Biosciences, Melbourne, Australia).

Techniques: Metabolomic, Expressing, Cell Characterization